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古朵新闻:实时定量,你真的做对了吗?
发布时间:2019-12-25   点击次数:66次

SHENGMINGTISHIYIGEDUOCENGCI,DUOGONGNENGDEFUZAJIEGOUTIXI,ZHUANLUZUDAIBIAOLEJIYINBIAODADEZHONGJIANZHUANGTAI。JIYINGONGNENGDEDUOZUXUEFENXIGUOCHENGZHONG,SHISHIDINGLIANGPCRJISHUPINGJIEQIDILIANDESHIYANCHENGBEN,BEIGUANGFANYINGYONGYUZHONGYAOGONGNENGJIYINBIAODACHAYIDEYANZHENG。YANJIURENYUANJINXUXIAZAIGenBankZHONGMUDEJIYINDECANZHAOmRNAXULIE,SHEJI、HECHENGYIDUIJIYINTEYIXINGKUOZENGYINWUHEYIDUISHIYIDEKANJIAJIYINKUOZENGYINWU,CHOUTIZONGRNA,FANZHUANLU,JINXINGSHISHIDINGLIANGPCRKUOZENG,TONGGUOBIJIAOSHIYANZUHEDUIZHAOZUJIANJIYINBIAODADECHAYI,JIUNENGQUEDINGSHIYANCHULIDUIGONGNENGJIYINBIAODADEYINGXIANG。DANSHIMAOSIFEICHANGCHENGSHUDESHIYANTIXIZHENDENENGJIESHITEDINGGONGNENGJIYINZHUANLUZUSHUIPINGBIAODADEZHENXIANGMA?YANJIURENYUANSHIFOUYILOULEMOUXIEKENENGYINGXIANGQIYANJIUJIELUNDESHENGWUXUESHIJIAN?

KEBIANJIANJIE(alternative splicing,AS,YOUCHENGXUANZEXINGJIANJIE,SHIZHENHESHENGWUJIYINBIAODADEPUBIANDIAOJIEJIZHI, ZHIGONGNENGJIYINTONGGUOBUTONGJIANJIE,CONGYIGEmRNAQIANTI(XUANZEBUTONGDEJIANJIEWEIDIANZUHE)CHANSHENGBUTONGDEmRNAJIANJIEYIGOUTIDEGUOCHENG。KEBIANJIANJIEKECHANSHENGZAIFEIFANYIQU(UTR)HUOBIANMAXULIE,QIJIZHIBAOKUO: WAIXIANZITIAOYUE(exon skip,ES)、NEIHANZIBAOLIU(retained intron,RI)、KEBIANGONGTIWEIDIAN(Alternate Donor site,AD)、KEBIANSHOUTIWEIDIAN(Alternate acceptor site,AA)、KEBIANQIDONGZI(Alternate promoter,AP)、KEBIANZHONGZHIZI(Alternate terminator,AT)HEWAIXIANZIHUCHI(Mutually exclusive exons, ME)ZHEIQILEI。mRNATONGGUOZHEIQILEIKEBIANJIANJIE,DUImRNADEWENDINGXING、DINGWEIHUOFANYIJINXINGDIAOKONG[1]。90%~95%DERENLEIJIYINHUIJINGLIXUANZEXINGJIANJIE[2,3]。20,000ZHONGRENLEIDANBAIZHIBIANMAJIYINZHONGYOU1/3YISHANGDEJIYINTONGGUOKEBIANJIANJIECHANSHENGDUOGEDANBAIYAXING[4]。CHULESHEJIQIGUANFAYU,YOUDAOXIBAOZENGZHI、XIBAOFENHUA,DIAOJIEXIBAOLEIXINGTEYIXINGGONGNENG[5、6]WAI,KEBIANJIANJIEHAIYUAIZHENG[7]、YAOWUBADIAN[8]DENGBINGLIGUOCHENGYOUGUAN。

彩搜网YILINSUANMEIHEJIDONGDANBAIDIAOJIEYINZI1(PHACTR1)WEILI,2009NIANGAIJIYINJIUBEIQUEDINGYURENGUANZHUANGDONGMAIJIBING(CAD)YOUGUAN[9]。JIEZHURACEHESHISHIDINGLIANGPCRJISHU,Valérie-AnneDENG(2018)QUEDINGGAIJIYINCUNZAI6ZHONGDANBAIYAXING,BAOKUODANHEXIBAOBIAODA435 bpDEDUANZHUANLUBEN(144 aa),DANAOBIAODA1743 bpDEZHANGZHUANLUBEN(580 aa),HEWAIXIANZI7、8HUO10、11KEBIANJIANJIECHANSHENGDEA+(1953bp,650 aa)、B+(1674 bp,557 aa)、A-(1746 bp,581 aa)、B- (1467 bp,488 aa)4ZHONGSHIZHONGZHANGDUDEZHUANLUBEN。CHUDUANZHUANLUBENYURENMIANYIGONGNENGYOUGUANWAI,ZHANGZHUANLUBENHEGUANZHUANGDONGMAINEIPIHUOXUEGUANPINGHUAJIXIBAONEIBIAODADE4ZHONGSHIZHONGZHANGDUZHUANLUBENJUNSHEJICAD。PHACTR1NEIHANZIDErs9349379WEIDIANKEPOHUAIMEF2DEJIEHEWEIDIAN,DIAOJIEA+、B+DEBIAODA,zuiZHONGYINGXIANGCAD[10]。

LINGWAI,MADECOBLL1JIYINCUNZAICOBLL1aHEWAIXIANZI9QUESHIDECOBLL1bZHEILIANGZHONGKEBIANJIANJIE。COBLL1bQUESHIDEWAIXIANZI9BIANMA40GEANJISUANCANJI,BAOHANLELAODANBAIJIMEI1、p38SILIEYUANHUOHUADANBAIJIMEIHEDANBAIJIMEICDELINSUANHUAWEIDIAN,ZHEIXIELINSUANHUAWEIDIANYUDANBAIDEWENDINGXINGMIQIEXIANGGUAN。TONGGUOBIJIAOsaiMADUOGEZUZHIDECOBLL1DEBIAODAFAXIAN,DUOGEZUZHIDECOBLL1bBIAODAJIAOWEIWENDING;SHENZANG、JISUI、FEIZHONGCOBLL1aDEBIAODALIANGzuiGAO,JIAZHUANGXIANHEJIECHANGZHONGBIAODAzuiDI;CHUNZHONGMAYUNDONGHOUJIROUZUZHICOBLL1aHECOBLL1bDEBIAODAJUNFASHENGXIADIAO。YINCI,COBLL1JIYINJINTONGGUOWAIXIANZI9DEYOUWUJIUSHIXIANLETONGYIJIYINZAIBUTONGZUZHIBIAODAMOSHIDEJINGXIDIAOKONG,BINGzuiZHONGYINGXIANGSAImaYUNDONGHOUDEJIROUPUTAOTANGSHUIPINGDEDIAOJIEHUONENGLIANGLAIYUANZHUANHUANGUOCHENG[11]。

彩搜网YISHANGLIANGGELIZIDOUSHUOMINGLE,TONGGUOKEBIANJIANJIE,TONGYIJIYINKEFAHUIBUTONGDESHENGWUXUEGONGNENG。RUGUOBULEJIEMUDEJIYINDEKEBIANJIANJIEQINGKUANG,KEYANRENYUANTONGGUOZHIJIEXIAZAIGanBankDENGLUDECANZHAOJIYINmRNAXULIE,JIUSHEJIGONGNENGJIYINDEDINGLIANGJIANCEYINWU,LAIJIANCEJIYINDEBIAODAQINGKUANG,HUODEDEHENKENENGSHIBUQUANMIAN,SHENZHICUOWUDEXINXI。

DUIGONGNENGJIYINBIAODADEZHENGQUEFENXI,SHOUXIANJIANLIZAIYANJIURENYUANDUIMUDEJIYINKEBIANJIANJIEDEQUANMIANRENSHIZHISHANG。SHOUXIAN,YANJIURENYUANYINGGAITONGGUORACEJISHUHUOSANDAIQUANZHANGZHUANLUZUCEXUJISHU,MINGQUEMUDEJIYINSHIFOUCUNZAIKEBIANJIANJIE。RUGUOZHENGSHIMUDEJIYINCUNZAIDUOZHONGKEBIANJIANJIEHOU,YINGDUIBUTONGKEBIANJIANJIEDEXULIEJINXINGBIDUIHESHENGWUXINXIXUEFENXI,QUERENKEBIANJIANJIEDELEIXINGHEBUTONGKEBIANJIANJIEDETEZHENGQUYU,ZHENDUIXIANGYINGTEZHENGQUYUSHEJIDINGLIANGDEJIANCEYINWU,CAINENGZHENGQUEHUOQUGONGNENGJIYINDEBIAODAQINGKUANG。

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