彩搜网

彩搜网 下载中心 > Western Blot详解(原理、试剂、步骤及问题解答)

下载中心

Western Blot详解(原理、试剂、步骤及问题解答)

点击次数:45 发布时间:2019/11/28
提 供 商: 上海古朵生物科技有限公司 资料大小:
图片类型: 下载次数: 0
资料类型: OCX 浏览次数: 45
相关产品:
详细介绍: 文件下载    

文件下载    

彩搜网WesternMIANYIYINJI(Western Blot)SHIJIANGDANBAIZHIZHUANYIDAOMOSHANG,RANHOULIYONGKANGTIJINXINGJIANCE。DUIYIZHIBIAODADANBAI,KEYONGXIANGYINGKANGTIZUOWEIYIKANGJINXINGJIANCE,DUIXINJIYINDEBIAODACHANWU,KETONGGUORONGHEBUFENDEKANGTIJIANCE。

彩搜网GUDUOSHENGWUZHUYAOTONGGUOYIXIAJIGEFANGMIANLAIXIANGXIDIJIESHAOYIXIAWestern BlotJISHU:

YI、 YUANLI

YU SouthernHUONorthernZAJIAOFANGFALEISI,DANWestern BlotCAIYONGDESHIJUBINGXIXIANANNINGJIAODIANYONG,BEIJIANCEWUSHIDANBAIZHI,“TANZHEN”SHIKANGTI,“XIANSE”YONGBIAOJIDEERKANG。JINGGUOPAGEFENLIDEDANBAIZHIYANGPIN,ZHUANYIDAOGUXIANGZAITI(LIRUXIAO SUANXIANWEISUBOMO)SHANG,GUXIANGZAITIYIFEIGONGJIAJIANXINGSHIXIFUDANBAIZHI,QIENENGBAOCHIDIANYONGFENLIDEDUOTAILEIXINGJIQISHENGWUXUEHUOXINGBUBIAN。

彩搜网YIGUXIANGZAITISHANGDEDANBAIZHIHUODUOTAIZUOWEIKANGYUAN,YUDUIYINGDEKANG TIQIMIANYIFANYING,ZAIYUMEIHUOTONGWEISUBIAOJIDEDIERKANGTIQIFANYING,JINGGUODIWUXIANSEHUOFANGSHEZIXIANYINGYIJIANCEDIANYONGFENLIDETEYIXINGMUDEJIYINBIAODADEDANBAICHENGFEN。GAIJISHUYEGUANGFANYINGYONGYUJIANCEDAN BAISHUIPINGDEBIAODA。

 

ER、FENLEI

彩搜网westernXIANSEDEFANGFAZHUYAOYOUYIXIAJIZHONG:

i. FANGSHEZIXIANYING

彩搜网ii. DIWUHUAXUEFAGUANGECL

彩搜网iii. DIWUYINGGUANGECF

iv. DIWUDABCHENGSE

彩搜网XIAN CHANGYONGDEYOUDIWUHUAXUEFAGUANGECLHEDIWUDABCHENGSE,TITONGSHUIPINGHESHIYANTIAOJIANDESHIYONGZHONGFANGFA,MUQIANFABIAOWENZHANGTONGCHANGSHIYONGDIWUHUAXUEFAGUANGECL。ZHIYAOMAIXIANCHENGDESHIJIHEJIUXING, CAOZUOYEBIJIAOJIANDAN,YUANLIRUXIA(ERKANGYONGHRPBIAOJI):FANYINGDIWUWEIGUOYANGHUAWU+LUMINUO,RUYUDAOHRP,JIFAGUANG,KESHIJIAOPIANPUGUANG,JIUKEXICHUTIAODAI。

SAN、ZHUYAOSHIJI

1、 BINGXIXIANANHEN,N’-YAJIASHUANGBINGXIXIANAN,YINGYIWENRE(YILIYURONGJIESHUANGBINGXIXIANAN)DEQULIZISHUIPEIZHIHANYOU29%(w/v)BINGXIXIANANHE1%(w/v)N,N’-YAJIASHUANGBING XIXIANANCHUCUNYEBINGXIXIANAN29g,N,N-YAJIACHASHUANGBINGXIXIANAN1g,JIAH2OZHI100ml。)CHUYUZONGSEPING,4℃BIGUANGBAOCUN。YANGEHESHIPHBUDECHAOGUO7.0,YINKEYIFA SHENGTUOANJIFANYINGSHIGUANGCUIHUAHUOJIANCUIHUADE。SHIYONGQIBUDECHAOGUOLIANGGEYUE,GEJIGEYUEXUZHONGXINPEIZHI。RUYOUCHENDIAN,KEYIGUOLV。

2、 SHIERWANJILIUSUANNASDSRONGYE:10%(w/v)0.1gSDS,1mlH2OQULIZISHUIPEIZHI,SHIWENBAOCUN。

3、 FENLIJIAOHUANCHONGYE:1.5mmol/LTris-HCL(pH8.8):18.15gTrisHE48ml1mol/LHCLHUNHE,JIASHUIXISHIDAO100mlZHONGTIJI。GUOLVHOU40CBAOCUN。

4、 NONGSUOJIAOHUANCHONGYE:0.5mmol/LTris-HCL(pH6.8):6.05gTrisRONGYU40mlH2OZHONG,YONGYUE48ml 1mol/L HCLDIAOZHIpH6.8JIASHUIXISHIDAO100mlZHONGTIJI。GUOLVHOU40CBAOCUN。ZHEILIANGZHONGHUANCHONGYEBIXUSHIYONGTrisJIANZHIBEI,ZAIYONGHCLDIAOJIEPHZHI,ERBUYONG Tris.CL。

彩搜网5、 TEMEDYUANRONGYEN,N,N’N’SIJIAJIYIERANCUIHUAGUOLIUSUANANXINGCHENGZIYOUJIERJIASULIANGZHONGBINGXIXIANANDEJUHE。PHTAIDISHI,JUHEFANYINGSHOUDAOYIZHI。10%(w/v)GUOLIUSUANANRONGYE。TIGONGLIANGZHONGBINGXIXIANANJUHESUOBIXUDEZIYOUJI。QULIZISHUIPEIZHISHUml,LINYONGQIANPEIZHI.

彩搜网6、 SDS-PAGEJIAYANGHUANCHONGYE:pH6.8 0.5mol/L TrisHUANCHONGYE8ml,GANYOU6.4ml,10%SDS 12.8ml,QIUJIYICHUN3.2ml,0.05%XIUFENLAN1.6ml,H2O 32mlHUNYUNBEIYONG。AN1:1HUO1:2BILIYUDANBAIZHIYANGPINHUNHE,ZAIFEISHUIZHONGZHU3minHUNYUNHOUZAISHANGYANG,YIBANWEI20-25ul,ZONGDANBAILIANG100μg。

7、 Tris-GANANSUANDIANYONGHUANCHONGYE:30.3gTris,188gGANANSUAN,10gSDS,YONGZHENGLIUSHUIRONGJIEZHI1000ml,DE0.25mol/L Tris-1.92mol/LGANANSUANDIANJIHUANCHONGYE。LINYONGQIANXISHI10BEI。

8、 ZHUANYIHUANCHONGYE:PEIZHI1LZHUANYIHUANCHONGYE,XUCHENGQU2.9gGANANSUAN、5.8gTrisJIAN、0.37g SDS,BINGJIARU200mlJIACHUN,JIASHUIZHIZONGLIANG1L。

彩搜网9、 LICHUNHONGRANYECHUCUNYE:LICHUNHONGS 2g SANLVyiSUAN30g HUANGJISHUIYANGSUAN 30g JIASHUIZHI100ml YONGSHISHANGSHUCHUCUNYEXISHI10BEIJICHENGLICHUNHONGSSHIYONGYE。SHIYONGHOUYINGYUYIFEIQI。

10、 TUOZHINAIFEN5%(w/v)。

11、 NaN3 0.02% DIEDANNA(YOUDU,DAISHOUTAOCAOZUO),RONGYULINSUANHUANCHONGYANRONGYE(PBS)。

12、 TrisHUANCHONGYANRONGYE(TBS):20mmol/LTris/HCL(pH7.5),500mmol/LnaCl。

彩搜网13、 Tween20(15)SHUKANGREN-MMP-9(16)SHUKANGREN-TIMP-1。

14、 GUOYANGHUAWUMEIBIAOJIDEDIERKANGTI。

彩搜网15、 NBT(RONGYU70%ERJIAJIJIAXIANAN,75mg/ml)。

彩搜网16、 BCIP(RONGYU100%ERJIAJIJIAXIANAN,50mg/ml)。

彩搜网17、 100mmol/LTris-HCL(pH9.5)。

彩搜网18、 100mmol/L NaCl。

19、 50mmol/LTris-HCL(pH7.5),5mmol/L EDTA。
(可以参看分子克隆)

 

 
网站彩搜网 公司简介 产品中心 招聘中心 技术支持 企业动态 联系我们
上海古朵生物科技有限公司版权所有 主营:优质胎牛血清,EA36染色液配制,迪夫快速染色液
GoogleSitemap ICP备案号:沪ICP备19048203号-2 技术支持:安防展览网
在线客服