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技术支持

几种常用的蛋白标签的功能和优点 古朵新闻√
发布时间:2019-12-09   点击次数:66次

ZHONGZUDANBAIBIAODAJISHUXIANYIJINGGUANGFANYINGYONGYUSHENGWUXUEGEGEJUTILINGYU。TEBIESHITINEIGONGNENGYANJIUHEDANBAIZHIDEDAGUIMOSHENGCHANDOUXUYAOYINGYONGZHONGZUDANBAIBIAODAZAITI。

MEIGUOGeneCopoeiaDEDANBAIBIAODAZAITIANZHAOBIAODASUZHUDEBUTONGXINTUICHU3LEI,FENBIEWEIBIAODASUZHUWEIDACHANGGANJUN,BURUDONGWUXIBAODE,YIJIMANBINGDUZAITI,SUZHUKEYIWEIBURUDONGWUXIBAOHEYUANDAIXIBAO。

CHULEBIYAODEFUZHIHESHAIXUANDEYUANJIAN,XIEZHUBIAODAHEFANYIDEYUANJIANWAI,BENWENJIANGGELEIZAITIFENBIEANZHAOGONGNENGBIAOQIANDEBUTONGQUEDINGZHONGLEIBINGJIANGGEBIAOQIANDEGONGNENGCHUBUJIESHAORUXIA:

His6:

His6SHIZHILIUGEZUANSUANCANJIZUCHENGDERONGHEBIAOQIAN,KECHARUZAIMUDEDANBAIDECMODUANHUONMODUAN。DANGMOUYIGEBIAOQIANDESHIYONG,YISHINENGGOUCHENGBIAOWEILIYUCHUNHUAHEJIANCE;ERSHIGOUCHENGDUTEDEJIEGOUTEZHENG(JIEHEPEITI)LIYUCHUNHUA。ZUANSUANCANJICELIANYUGUTAIDENIEYOUQIANGLIEDEXIYINLI,KEYONGYUGUDINGHUAJINSHUAOHECENGXI(IMAC),DUIZHONGZUDANBAIJINXINGFENLICHUNHUA。

SHIYONGHis-tagYOUXIAMIANYOUDIAN:

1.BIAOQIANDEFENZILIANGXIAO,ZHIYOU~0.84KD,ERGSTHEDANBAIAFENBIEWEI~26KDHE~30KD,YIBANBUYINGXIANGMUBIAODANBAIDEGONGNENG;

2.HisBIAOQIANRONGHEDANBAIKEYIZAIFEILIZIXINGBIAOMIANHUOXINGJICUNZAIDETIAOJIANXIAHUOBIANXINGTIAOJIANXIACHUNHUA,QIANZHEZAICHUNHUASHUSHUIXINGQIANGDEDANBAIDEDAOYINGYONG,HOUZHEZAICHUNHUABAOHANTIDANBAISHITEBIEYOUYONG,YONGGAONONGDUDEBIANXINGJIRONGJIEHOUTONGGUOJINSHUAOHEQINHECENGXIQUCHUZADANBAI,SHIFUXINGBUSHOUQITADANBAIDEGANRAO,HUOJINXINGJINSHUAOHEQINHECENGXIFUXING;

彩搜网3.HisBIAOQIANRONGHEDANBAIYEBEIYONGYUDANBAIZHI-DANBAIZHI、DANBAIZHI-DNAXIANGHUZUOYONGYANJIU;

彩搜网4.HisBIAOQIANMIANYIYUANXINGXIANGDUIJIAODI,KEJIANGCHUNHUADEDANBAIZHIJIEZHUSHEDONGWUJINXINGMIANYIZHIBEIKANGTI;

彩搜网5.KEYINGYONGYUDUOZHONGBIAODAXITONG,CHUNHUADETIAOJIANWENHE;

彩搜网6.KEYIHEQITADEQINHEBIAOQIANYIQIGOUJIANSHUANGQINHEBIAOQIAN。

Flag:

FlagBIAOQIANDANBAIWEIBIANMA8GEANJISUANDEQINSHUIXINGDUOTAI(DYKDDDDK),TONGSHIZAITIZHONGGOUJIANDEKozakXULIESHIDEDAIYOUFLAGDERONGHEDANBAIZAIZHENHEBIAODAXITONGZHONGBIAODAXIAOLVGENGGAO。

彩搜网FLAGZUOWEIBIAOQIANDANBAI,QIRONGHEBIAODAMUDEDANBAIHOUJUYOUYIXIAYOUDIAN:

彩搜网1.FLAGZUOWEIRONGHEBIAODABIAOQIAN,QITONGCHANGBUHUIYUMUDEDANBAIXIANGHUZUOYONGBINGQIETONGCHANGBUHUIYINGXIANGMUDEDANBAIDEGONGNENG、XINGZHI,ZHEIYANGJIUYOULIYONGYANJIURENYUANDUIRONGHEDANBAIJINXINGXIAYOUYANJIU。

彩搜网2.RONGHEFLAGDEMUDEDANBAI,KEYIZHIJIETONGGUOFLAGJINXINGQINHECENGXI,CICENGXIWEIFEIBIANXINGCHUNHUA,KEYICHUNHUAYOUHUOXINGDERONGHEDANBAI,BINGQIECHUNHUAXIAOLVGAO。

3.FLAGZUOWEIBIAOQIANDANBAI,QIKEYIBEIKANGFLAGDEKANGTISHIBIE,ZHEIYANGJIUFANGBIANTONGGUOWestern Blot、ELISADENGFANGFADUIHANYOUFLAGDERONGHEDANBAIJINXINGJIANCE、JIANDING。

彩搜网4.RONGHEZAINDUANDEFLAG,QIKEYIBEICHANGJIMEIQIECHU(DDDK),CONGERDEDAOTEYIDEMUDEDANBAI。YINCIXIANFLAGBIAOQIANYIGUANGFANDEYINGYONGYUDANBAIBIAODA、CHUNHUA、JIANDING、GONGNENGYANJIUJIQIDANBAIXIANGHUZUOYONGDENGXIANGGUANLINGYU。

MBP:

MBP(MAIYATANGJIEHEDANBAI)BIAOQIANDANBAIDAXIAOWEI40kDa,YOUDACHANGGANJUNK12DEmalEJIYINBIANMA。MBPKEZENGJIAZAIXIJUNZHONGGUOLIANGBIAODADERONGHEDANBAIDERONGJIEXING,YOUQISHIZHENHEDANBAI。MBPBIAOQIANKETONGGUOMIANYIFENXIHENFANGBIANDIJIANCE。YOUBIYAOYONGWEIDIANZHUANYIDEDANBAIMEIQIEGEBIAOQIAN。RUGUODANBAIZAIXIJUNZHONGBIAODA,MBPKEYIRONGHEZAIDANBAIDENDUANHUOCDUAN。

CHUNHUA:RONGHEDANBAIKETONGGUOJIAOLIANDIANFENQINHECENGXIYIBUCHUNHUA。JIEHEDERONGHEDANBAIKEYONG10mMMAIYATANGZAISHENGliHUANCHONGYEZHONGJINXINGXITUO。JIEHEQINHELIZAIWEIMOERFANWEI。YIXIERONGHEDANBAIZAI0.2% Triton X-100HUO0.25% Tween 20CUNZAIXIABUNENGYOUXIAOJIEHE,ERQITARONGHEDANBAIZEBUSHOUYINGXIANG。 HUANCHONGTIAOJIANWEIpH7.0DAO8.5,YANNONGDUKEGAODA1M,DANBUNENGSHIYONGBIANXINGJI。RUGUOYAOQUCHUMBPRONGHEBUFEN,KEYONGWEIDIANTEYIXINGDANBAIMEIQIECHU。

JIANCE:KEYONGMBPKANGTIHUOBIAODADEMUDEDANBAITEYIXINGKANGTIJIANCE。

GST:

GST(GUGUANGGANTAIQIUJIZHUANYIMEI) BIAOQIANDANBAIBENSHENSHIYIGEZAIJIEDUGUOCHENGZHONGQIDAOZHONGYAOZUOYONGDEZHUANYIMEI,TADETIANRANDAXIAOWEI26KD。JIANGTAYINGYONGZAIYUANHEBIAODADEYUANYINDAZHIYOULIANGGE,YIGESHIYINWEITASHIYIGEGAODUKERONGDEDANBAI,XIWANGKEYILIYONGTAZENGJIAWAIYUANDANBAIDEKERONGXING;LINGYIGESHITAKEYIZAIDACHANGGANJUNZHONGDALIANGBIAODA,QIDAOTIGAOBIAODALIANGDEZUOYONG。GSTRONGHEBIAODAXITONGGUANGFANYINGYONGYUGEZHONGRONGHEDANBAIDEBIAODA,KEYIZAIDACHANGGANJUNHEJIAOMUJUNDENGSUZHUXIBAOZHONGBIAODA。JIEHEDERONGHEDANBAIZAIFEIBIANXINGTIAOJIANXIAYONG10mM HAIYUANXINGGUGUANGGANTAIXITUO。ZAIDADUOSHUQINGKUANGXIA,RONGHEDANBAIZAISHUIRONGYEZHONGSHIKERONGDE,BINGXINGCHENGERTI。GSTBIAOQIANKEYONGMEIXUEFENXIHUOMIANYIFENXIHENFANGBIANDEJIANCE。BIAOQIANYOUZHUYUBAOHUZHONGZUDANBAIMIANSHOUBAOWAIDANBAIMEIDEJIANGJIEBINGTIGAOQIWENDINGXING。ZAIDADUOSHUQINGKUANGXIAGSTRONGHEDANBAISHIWANQUANHUOBUFENKERONGDE。

CHUNHUA:GAIBIAODAXITONGBIAODADEGSTBIAOQIANDANBAIKEZHIJIECONGXIJUNLIEJIEYEZHONGLIYONGHANYOUHAIYUANXINGGUGUANGGANTAIQIONGZHITANGNINGJIAO(Glutathionesepharose)QINHESHUZHIJINXINGCHUNHUA。GSTBIAOQIANDANBAIKEZAIWENHE、FEIBIANXINGTIAOJIANXIAXITUO,YINCIBAOLIULEDANBAIDEKANGYUANXINGHESHENGWUHUOXING。GSTZAIBIANXINGTIAOJIANXIAHUISHIQUDUIGUGUANGGANTAISHUZHIDEJIEHENENGLI,YINCIBUNENGZAICHUNHUAHUANCHONGYEZHONGJIARUQIANGBIANXINGJIRU:YANSUANGUAHUONIAOSUDENG。

RUGUOYAOQUCHUGSTRONGHEBUFEN,KEYONGWEIDIANTEYIXINGDANBAIMEIQIECHU。

彩搜网JIANCE:KEYONGGSTKANGTIHUOBIAODADEMUDEDANBAITEYIXINGKANGTIJIANCE。

HA:

HABIAOQIANDANBAI,BIAOQIANXULIEYPYDVPDYA,YUANYULIUGANBINGDUDEHONGXIBAONINGJISUBIAOMIANKANGYUANJUEDINGCU,9GEANJISUAN,DUIWAIYUANBADANBAIDEKONGJIANJIEGOUYINGXIANGXIAO, RONGYIGOUJIANCHENGBIAOQIANDANBAIRONGHEDAONDUANHUOZHECDUAN。YIYUYONGAnti-HAKANGTIJIANCEHEELISAJIANCE。

c-Myc:

彩搜网C-MycBIAOQIANDANBAI,SHIYIGEHAN11GEANJISUANDEXIAOBIAOQIAN,BIAOQIANXULIEGlu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu,ZHEI11GEANJISUANZUOWEIKANGYUANBIAOWEIBIAODAZAIBUTONGDEDANBAIZHIKUANGJIAZHONGRENGKESHIBIEQIXIANGYINGKANGTI。C-Myc tagYICHENGGONGYINGYONGZAI Western-blotZAJIAOJISHU、MIANYICHENDIANHELIUSHIXIBAOJILIANGSHUZHONG, KEYONGYUJIANCEZHONGZUDANBAIZHIZAIBAXIBAOZHONGDEBIAODA。

彩搜网eGFP/eCFP/eYFP/mCherry:

彩搜网FENBIESHIZENGQIANGXINGLVSEYINGGUANGDANBAI/ZENGQIANGXINGHUANGLVSEYINGGUANGDANBAI/ZENGQIANGXINGHUANGLVSEYINGGUANGDANBAI/DANTIHONGSEYINGGUANGDANBAI,JUYOUBUTONGDEJIFABOZHANGFASHEBOZHANGWEI,JUNYOUYESHENGXINGYINGGUANGDANBAITONGGUOANJISUANTUBIANHEMIMAZIYOUHUAERLAI。JIUeGFPERYAN,XIANGDUIYUGFP,QIYINGGUANGQIANGDUGENGQIANG、YINGGUANGXINGZHIGENGWENDING。TONGSHIZAITIZHONGGOUJIANDEKozakXULIESHIDEHANYOUeGFPDERONGHEDANBAIZAIZHENHEBIAODAXITONGZHONGBIAODAXIAOLVGENGGAO。mCherrySHICONGDsRedYANHUALAIDEXINGNENGzuiHAODEYIGEDANTIHONGSEYINGGUANGDANBAI,KEYIHEGFPXILIEYINGGUANGDANBAIGONGYONG,SHIXIANDUOSEBIAOJITINEI、WAISHIYANBIAOMING,mCherryZAINDUANHECDUANRONGHEWAIYUANDANBAISHI,YINGGUANGDANBAIHUOXINGHEBEIRONGHEDEMUBIAODANBAIGONGNENGXIANGHUMEIYOUMINGXIANYINGXIANG。

ZHEIXIEYINGGUANGBIAOQIANDANBAI,QIRONGHEBIAODAMUDEDANBAIHOUJUYOUYIXIAYOUDIAN:

1.BUYONGPOSUIZUZHIXIBAOHEBUJIARENHEDIWU,ZHIJIETONGGUOYINGGUANGXIANWEIJINGJIUNENGZAIHUOXIBAOZHONGFACHULVSEYINGGUANG,SHISHIXIANSHIMUDEJIYINDEBIAODAQINGKUANG,ERQIEYINGGUANGXINGZHIWENDING,BEIYUWEIHUOXIBAOTANZHEN。

2.QIZIFAYINGGUANG,BUXUYONGMUDEJIYINDEKANGTIHUOYUANWEIZAJIAOJISHUJIUKETUIZHIMUDEJIYINZAIXIBAOZHONGDEDINGWEIDENGQINGKUANG。

3.TONGSHIXIBAONEIDEQITACHANWUBUHUIGANRAOBIAOQIANDANBAIJIANCE,CONGERSHIQIJIANCEGENGXIANDEKUAISU、JIANBIAN、LINGMINDUGAOERQIEZHONGXIANXING。

彩搜网4.QIDIXIAOHAO、GAOLINGMINDUJIANCE,SHIFENSHIYONGYUGAOTONGLIANGDEYAOWUSHAIXUAN。YINCIXIANeGFP BIAODABIAOQIANBEIGUANGFANDIYINGYONGYUJITUANBIAODADIAOKONG、ZHUANJIYINGONGNENGYANJIU、DANBAIZAIXIBAOZHONGDEGONGNENGDINGWEI、QIANYIBIANHUAJIYAOWUSHAIXUANDENGFANGMIAN。

彩搜网5.mCherryHONGSEYINGGUANGDANBAIZAIBIAOJIBINGDUKELI,YANJIUBINGDUHESUZHUXIBAOZHIJIANGENGYOUDETIANDUHOUDEYOUSHI。RUYONGmRFPHUOmCherryBIAOJIHIVBINGDUKELI,YANJIUH1VHESUZHUXIBAOWENDEXIANGHUZUOYONGYIJIHIVQINRANSUZHUXIBAODEGUOCHENG.YONGmRFPBIAOJIMANBINGDUKELI,YANJIUMANBINGDUZAIXIAOSHUTINEIDEFUZHIGUOCHENGDENG。

YINGGUANGSUMEI:

彩搜网LAIYUANYUSHENGWUTINEIDEYINGGUANGSU,CHANGJIANDEYOUYINGHUOCHONGYINGGUANGSUMEI、HAISHENYINGGUANGSUMEIHEGuassiaYINGGUANGSUMEI。ZHEIXIEYINGGUANGSUMEIZUOWEI“BAOGAODANBAI”BEIYONGYUFENZISHENGWUXUEYANJIUZHONG,ZHEIZHONGJISHUBEICHENGWEIBAOGAOJIYINJIANCEFAHUOYINGGUANGSUMEIJIANCEFA(Luciferase Assay)。GENPUTONGRONGHEDANBAIBIAOQIANBUTONG,SHIYONGYINGGUANGSUMEIGOUJIANDEBAOGAOJIYINKEYONGZUOMUDEJIYINDEDINGLIANGFENXI。YINCICHANGYONGYUYANJIUQIDONGZI、miRNA 3'UTRKELONGDEGONGNENGYUDIAOKONG,YINWEITAMENDUIMUDEJIYINDEDIAOKONGKEYISHIJIANBIANDE,ERBUSHIJIANDANDEKAIHEGUANLIANGZHONGZHUANGTAI。

YOUDIAN:LINGMINDUGAO,JIANCEFUDUKUAN;BUSHIBURUDONGWUXIBAONEIYUANXINGJIYIN;ZHONGFUXINGHAO;YUHTSJIANRONGDENG。YINGHUOCHONGHEHAISHENYINGGUANGSUMEIYIJINGBEIGUANGFANYINGYONGYUXIETONGBAOGAOBINGJINXINGJUNYIHUAYANJIU。YOUYUTAMENDOUSHIKUAISU,RONGYIHEGAOLINGMINDEJIANCEFANGFA。ERQIEYINGHUOCHONGHEHAISHENYINGGUANGSUMEISHILIXIANGDESHUANGJIYINBAOGAOXITONG,YINWEITAMENLAIYUANYUBUTONGDESHENGWUJINHUAFANGXIANG,DANBAIJIEGOUHEDIWUCHAYIDOUHENDA,ZAISHIYANZHONGBUHUICHANSHENGXIANGHUYINGXIANG。

JIYUYINGGUANGSUMEIDETEXING,WOSIYANFATIGONGDESecrete-Pair? Dual Luminescence Assay KitKEYONGYUFENXISHUANGBAOGAOXITONGZHONGGaussiaYINGGUANGSUMEI(GLuc)HEFENMIXINGJIANXINGLINSUANMEI(SEAP)HUOXING。GLuc HESEAPJUNWEIFENMIXINGBAOGAODANBAI,WUXULIEJIEXIBAOJIKEBIANJIEDECONGXIBAOPEIYANGYEZHONGQUDEYANGBEN,BINGDUISHIYANJIEGUOJINXINGJINGZHUNDESHISHIFENXI。

Avi Tag:

AviTagBIAOQIANDANBAISHIYIGE15 GEANJISUANDEDUANTAI,JUYOUYIGEDANSHENGWUSUHUALAIANSUANWEIDIAN,YUYIZHITIANRANKESHENGWUSUHUAXULIEWANQUANBUTONG,KEYIJIAZAIMUBIAODANBAIDENDUANHECDUAN。RONGHEBIAODAHOU,KEBEISHENGWUSULIANJIEMEISHENGWUSUHUA,WEILECHUNHUAZHONGZUDANBAIXUANYONGDIQINHEXINGDEDANTIKANGSHENGWUSUDANBAIHUOKANGSHENGWUSUDANBAIYANSHENGWU,CHULEYONGYUDANBAIZHIFENLICHUNHUA,HAIYONGYUDANBAIZHIXIANGHUZUOYONGYANJIU。

Avi TagBIAOQIANXITONGJUYOUYIXIAJIDAYOUDIAN:

彩搜网1.WULUNZAITIWAIHUOZHETINEI,JIHUSUOYOUDEDANBAIDOUKEYIZAIYIGEDUTEDEAvi TagWEIDIANQINGYIQIEYOUXIAODIBEISHENGWUSUHUA;

2.SHENGWUSUHUASHITONGGUOMEIHEDIWUDEFANYINGLAISHIXIAN,FANYINGTIAOJIANXIANGDANGWENHEERQIEBIAOJIDEZHUANYIXINGJIGAO;

彩搜网3.SHENGWUSUAviTagZHIYOU15GEANJISUAN,DUIDANBAIKONGJIANJIEGOUDEYINGXIANGFEICHANGXIAO。

SNAP-Tag:

彩搜网SNAP-TagSHIXINYIDAIDEDANBAIBIAOQIANJISHU,BUJINZHUANYIXINGJIGAOERQIEWENDING,zuiDADEYOUDIANSHISHIYONGYUDUOZHONGHUANJINGXIADEDANBAIZHIJIANCEYUCHUNHUA,RUHUOXIBAONEI、RONGYEZHONG、HUOGUTAIXIANG(RUSDS-PAGE gels)DENG。

SNAP-TagSHICONGRENDEO6-JIAJINIAOPIAOLING-DNAJIAJIZHUANYI(O6-alkylguanine-DNA-alkyltransferase)HUODE。WULUNTINEIHAISHITIWAI,SNAP-TagDOUNENGYUDIWUGAOTEYIXINGDIGONGJIAJIEHE,SHIDANBAIBIAOJISHANGSHENGWUSUHUOYINGGUANGJITUAN(RUYINGGUANGSUHERUODANMING)。SNAPSUODAIDEHUOXINGQIUJIWEIDIANJIESHOULEBENJIAJINIAOPIAOLINGSUOXIEDAIDECELIANBENJIAJIJITUAN,SHIFANGCHULENIAOPIAOLING。ZHEIZHONGXINDELIUMIJIANGONGJIAJIEHESHISNAPSUODAIDEMUDEDANBAIXIEDAISHANGLEBENJIAJIJITUANSUODAIDEBIAOJIWU。BENJIAJINIAOPIAOLINGZAISHENGHUATIAOJIANXIAWENDING,BINGQIEMEIYOUQITADANBAIHUIHEZHEILEIWUZHIZUOYONG,SUOYISNAPBIAOQIANFANYINGSHIGAOTEYIDE。

JIANCE:SHENGWUSUHUOGEZHONGYANSEYINGGUANGDEDIWU(RUYINGGUANGSU、RUODANMING)KESHENTOUJINRUXIBAO,FANGBIANKUAIJIEDIJINXINGHUOXIBAONEISNAP-TagRONGHEDANBAIDEBIAOJIYUJIANCE。TAMENYEKETEYIXINGDIBIAOJIDACHANGGANJUN,JIAOMUHEBURUDONGWUDENGXIBAOCHOUTIYEHUOYIJINGCHUNHUADEDANBAIYEZHONGDESNAP-tagRONGHEDANBAI。

彩搜网JIANGCHUNHUADEHUOWEICHUNHUADESNAP-TagRONGHEDANBAIYUBIAOMIANGUDINGLEBENJIAJINIAOPIAOLINGDEJIZHIHUNHE,DANBAIJIKETEYIYUDIWUZUOYONG,XINGCHENGGONGJIAJIAN,RONGHEDANBAIJIANJIEBEIGUDINGZAILEJIZHIBIAOMIANSHANG,KEYIDADAOGENGFANGBIANKUAIJIEDIYANJIUDANBAIGONGNENGHUOCHUNHUADANBAIDEMUDE。

Halo Tag:

彩搜网HaloTag?BIAOQIANDANBAISHIYIZHONGTUOLUSUMEIDEYICHUANXIUSHIYANSHENGWU,KEYUDUOZHONGHECHENGDEHaloTag?PEIJIYOUXIAODIGONGJIAJIEHE。ZHEIGEFENZILIANGWEI33KDaDEDANTIDANBAINENGRONGHEZAIZHONGZUDANBAIDENDUANHUOC DUAN,BINGZAIYUANHEHEZHENHEXITONGZHONGBIAODA。

彩搜网HaloTag?PEIJISHIXIAOFENZIHUAXUEWU,NENGGOUZAITIWAIHUOTINEIYUHaloTag?DANBAIGONGJIAJIEHE。ZHEIXIEPEIJIYOULIANGGEGUANJIANZUFENZUCHENG:(1)YIGETONGYONGDEHaloTag?FANYINGLIANJIEZI,JIEHEHaloTag?DANBAI;(2)YIGEGONGNENGJITUAN,LIRUYINGGUANGRANLIAOHUOQINHEMEIJIE。

彩搜网NENGGOUGONGJIAHETEYIXINGJIEHEDUOZHONGHECHENGDEBAOGAOJITUANHEQINHEPEIJIDETEXING,SHIDEHaloTag?DANBAINENGGOUYONGYUJIANCEHEQINHEJIEHEHUOGUXIANGHUAGUDINGMUDEDANBAI。

SUMO:

SUMOBIAOQIANDANBAISHIYIZHONGXIAOFENZIFANSUYANGXIUSHIDANBAI(Small ubiquitin-likemodifier),SHIFANSU(ubiquitin)LEIDUOTAILIANCHAOJIAZUDEZHONGYAOCHENGYUANZHIYI。ZAIYIJIJIEGOUSHANG,SUMOYUFANSUZHIYOU18%DETONGYUANXING,RANERLIANGZHEDESANJIJIEGOUJIQISHENGWUXUEGONGNENGQUESHIFENXIANGSI。

YANJIUFAXIANSUMOKEYIZUOWEIZHONGZUDANBAIBIAODADERONGHEBIAOQIANHEFENZIBANLV,BUDANKEYIJINYIBUTIGAORONGHEDANBAIDEBIAODALIANG,QIEJUYOUKANGDANBAIMEISHUIJIEYIJICUJINBADANBAIZHENGQUEZHEDIE,TIGAOZHONGZUDANBAIKERONGXINGDENGGONGNENG。

彩搜网CIWAISUMOHAIYOUYIXIANGZHONGYAODEYINGYONG,JIUSHIKEYONGYUWANZHENGDIQIECHUBIAOQIANDANBAI,DEDAOTIANRANDANBAI。YINWEISUMODANBAISHUIJIEMEINENGSHIBIEWANZHENGDESUMOBIAOQIANDANBAIXULIE,BINGNENGGAOXIAODIBASUMOCONGRONGHEDANBAISHANGQIEGEXIALAI。QIECHUSUMOHOU,JINGGUOQINHECENGXI,QUCHUBIAOQIANDANBAIBUFEN,JIUDEDAOHETIANRANDANBAIYIYANGDEZHONGZUDANBAI。SUOYISUMOBIAOQIANYECHANGYONGYUHEQITABIAOQIANYIQIYINGYONG,ZUOWEITEYIMEIQIESHUIJIEWEIDIAN。

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