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使用慢病毒的常见问题 古朵新闻√
发布时间:2019-04-11   点击次数:148次

1. 怎样购买份量合适数量的慢病毒颗粒?

彩搜网YITAOWANZHENGSHIYANSUOXUDEMANBINGDUKELIBAOKUO:1. HANYOUMUDEJIYINDEMANBINGDUKELI,2. YINXINGDUIZHAOMANBINGDUKELI,3. YONGYUYUSHIYANDEYANGXINGDUIZHAOMANBINGDUKELI。

GOUMAISHIKEYIGENJUMUDEXIBAOTEXING、JIANCEFANGFA、PEIYANGQIMINGUSUANMANBINGDUKELIDEzuiJIAYONGLIANG,BIMIANSHENGYUDEDALIANGMANBINGDUKELICHAOCHUzuiJIABAOCUNQI;CIWAI,GeneCopoeiaTM TONGSHITIGONGGAODIDUDIJIAGEDEYANGXINGDUIZHAOMANBINGDUKELI,BANGZHUNINYIJIAODIDEYUSUANHEJIAOCHONGZUDECAILIAOWANCHENGYUSHIYANDETIAOJIANTANSUO。CHUCISHIYONGMANBINGDUKELIDEYANJIUZHEYEKESHIYONGYANGXINGDUIZHAOMANBINGDUKELISHUXISHIYANGUOCHENG。

 

2. 慢病毒颗粒可以用于动物体内(In vivo)实验吗?

彩搜网GeneCopoeiaTM TIGONGDEJIYONGXINGMANBINGDUKELIQUANBUJINGGUOCHUNHUA、NONGSUOCHULI,QUCHULEXIBAOSUIPIANDENGZAZHI,JINYIBUJIANGDIMIANYIYUANXING,SHIYONGYUGELEIHUOTIDONGWUZHUSHESHIYANHECHENGLIUSHIYAN。

彩搜网3. MANBINGDUKELIDUIMUDEXIBAODEGANRANXIAOLVHENDI,RUHETIGAOGANRANXIAOLV?

TIGAOGANRANXIAOLVDEQIANTISHIBAOZHENGXIBAOSHENGZHANGZHUANGTAILIANGHAO。QICI,KEYITONGGUOTIGAO MOI ZHILAITIGAOGANRANXIAOLV,YEKEYIZAIPEIYANGJIZHONGJIARU Polybrene (4-10 ?g/mL) TIGAOGANRANXIAOLV。

 

4. 添加慢病毒颗粒后,细胞为什么大量死亡?

彩搜网MANBINGDUKELIDUIBAXIBAOYOUYIDINGDUXING。TIANJIALIANGGUODUO、GANRANSHIJIANGUOZHANGDOUKENENGDUIMUDEXIBAO ZAOCHENGSHANGHAI。RUYUSHANGZHEIZHONGQINGKUANG,JIANYININJIANGDI MOI ZHI,BINGZAIGANRANXIBAO 4-8 XIAOSHIHOUJINXINGHUAN YE(YIXINXIANDEQUANPEIYANGJITIHUANHANMANBINGDUKELIDEJIUPEIYANGJI),ZHANGHUANYESHIJIANBUKEDAYU 12 XIAOSHI。

 

5.Polybrene 是什么?在慢病毒颗粒感染中,Polybrene 添加越多越好吗?

彩搜网Polybrene SHICHANGYONGDEGANRANTIANJIAJI,TONGCHANGDESHIYONGNONGDUWEI 4-10 ?g/mL,Polybrene NENG XIANZHUTIGAOMANBINGDUDEGANRANXIAOLV,TONGCHANGNENGTIGAOGANRANXIAOLV 2-10 BEI,DUIBUFENXIBAOSHENZHIKE TIGAOGANRANXIAOLV 10-20 BEI。DANGMUDEXIBAO MOI GAOYU 20 SHI,WOMENJIANYIZAIPEIYANGJIZHONGJIARU Ploybrene(YUE 4-10 ?g/mL) SHIDANGTIGAOGANRANXIAOLV。

RANER,Polybrene YOUYIDINGDEXIBAODUXING,BUTONGXIBAODUI Polybrene DEMINGANDUHENAISHOUXINGBUTONG,BUFENXIBAODUI Polybrene FANYINGMINGXIAN,RONGYIDAOZHIXIBAOZHUANGTAIBIANCHA、XINGTAIFASHENGBIANHUA、SHENZHISIWANG,YINCIBINGFEIMEIZHONGXIBAODOUSHIHETIANJIA Polybrene。

彩搜网ZAIZHENGSHISHIYONG Polybrene QIAN,JIANYIZAI 1-10 ?g/mL FANWEINEISHEZHITIDU,GUANCHAXIBAOZAIGAINONGDUXIA,24 XIAOSHIHOUSHIFOURENGBAOCHIJIANKANGSHENGZHANG,CONGERQUEDINGGAIXIBAODESHI Polybrene NONGDU。

 

6. 目的细胞可以被慢病毒颗粒感染,但 eGFP 的荧光强度很低,为什么?

彩搜网ZAIMUDEXIBAOBEIMANBINGDUKELIGANRANGUOCHENGZHONG,eGFP YINGGUANGQIANGDUQUJUEYUBINGDUGANRANXIBAONEIDEMANBINGDUKELISHU、XIBAOBENSHENDEZHUANGTAI、XIBAOLEIXINGYIJIGUANCHASHIJIANDENG。YIBANQINGKUANGXIA,MUDEXIBAOGANRANMANBINGDUKELISHUYUEDUO,XIBAOZENGZHIYUEKUAI,eGFP YINGGUANGHUIJIAOQIANG。MANBINGDUXIEDAIJIYINBIAODASHIJIANJIAOZHANG,YIBANZAIZENGZHIJIAOKUAIDEXIBAOZHONGYEXUYAOGANRAN 72-96 XIAOSHICAIHUIDAODA eGFP DEBIAODAGAOFENG。DUIYUZENGZHIJIAOMANDEXIBAO,GANRANSHIJIANZEXUGENGZHANG。JIANYIRUGUONINDEXIBAOZAIGANRANHOUGUANCHADEYINGGUANGQIANGDUJIAODI,JIANYIJIXUPEIYANGYIDUANSHIJIANZAIGUANCHA。

 

7. 进行慢病毒颗粒感染后,为什么目的细胞用 qPCR 检测不到目的基因表达量的变化?

彩搜网RUGUOSHIYONGDEJIANCEFANGFASHI qPCR,YUANYINKENENGSHIMUDEJIYINHANTESHUXULIEJIEGOU、HUOYUMUDEXIBAOXITONGBUNENGJIANRONGDEWENTI,DAOZHIZHUANLUSHOUYINGXIANG,JIANYITONGSHIPEIYANG、GANRANMUDEXIBAOHE 293T GONGJUXIBAO,TONGSHIJINXINGJIANCE,RUMUDEJIYINZAI 293T XIBAOZHUANLUBUSHOUYINGXIANG,ZEKENENGWEIMUDEJIYINYUMUDEXIBAODEXIANGHUZUOYONGDAOZHIZHUANLUSHOUZU。

 

8. 慢病毒颗粒在储存期间不慎染菌,还可以继续使用吗?

彩搜网RUMANBINGDUKELIBUSHENRANJUN,QIESHIYANCAILIAOYOUXIAN,KEYISHIYONG 0.45 ?m LVMODUIMANBINGDUJINXINGLVJUNCAOZUO。DANGRAN,GAICAOZUOHUIDAOZHIYIDINGCHENGDUDEDIDUSUNSHIJIMANBINGDUKELIZONGLIANGSUNSHI。RUTIAOJIANYUNXU,JIANYIZHONGXINGOUMAIGAIZHONGMANBINGDU。

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